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2 edition of Biosynthesis of folate polyglutamates in the rat. found in the catalog.

Biosynthesis of folate polyglutamates in the rat.

John Bates

Biosynthesis of folate polyglutamates in the rat.

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Published by University of Aston. Department of Chemistry in Birmingham .
Written in English


Edition Notes

Thesis (Ph.D.) - University of Aston in Birmingham 1981.

ID Numbers
Open LibraryOL13773733M

Full text of "Militia and Survival Big Books Collection" See other formats. and the current book, Sports Nutrition: Vitamins and Trace Elements, 2nd edition, all with Ira Wolinsky. She has published more than refereed research articles and 12 book chapters, as well as several publications intended for lay audiences, and has given numerous presentations to professional and .


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Biosynthesis of folate polyglutamates in the rat. by John Bates Download PDF EPUB FB2

Fig. FOLATE CHEMISTRY AND NOMENCLATURE. Folic acid (pteroylmonoglutamate [PteGlu]) is the commercially available parent compound for more than compounds collectively referred to as folates.

PteGlu consists of three basic components: a pteridine derivative, a p-aminobenzoic acid residue, and an l-glutamic acid PteGlu can play a role as a coenzyme, it must first be.

In contrast, all animals receiving a daily supplement of folate rice or folic acid fortified rice (on average μg/rat/day and μg/rat/day, respectively) and those receiving a positive.

It provides efficient deconjugation of folate polyglutamates to monoglutamates without compromising stability because the pHoptimum of rat plasma conjugase is (Horne et al., ).

Therefore, the deconjugation of folate polyglutamates is an essential prerequisite for reliable results in the folate analysis. Omitting this step may easily lead to underestimated and even false negative analytical results in both MA and HPLC methods. Rat serum/plasma is commonly used as a source of folate conjugase in many current by: 1.

Abstract. Folate is a crucial intermediate for one-carbon metabolism. In higher plants, folate derivatives are found in the cytosol, mitochondria and, to a lesser extent, chloroplasts together with the enzymes serine hydroxymethyltransferase and methylenetetrahydrofolate reductase/cyclohydrolase required for the generation and interconversion of the one-carbon substituted by: Folate functions as a coenzyme in single-carbon transfers in the metabolism of nucleic and amino acids.

The primary indicator used to estimate the Recommended Dietary Allowance (RDA) for folate is erythrocyte folate in conjunction with plasma homocysteine and folate concentrations. The RDA for both men and women is µg/day of dietary folate.

Folate functions as a coenzyme in single-carbon transfers in the metabolism of nucleic and amino acids. The primary indicator used to estimate the Recommended Dietary Allowance (RDA) for folate is erythrocyte folate in conjunction with plasma homocysteine and folate concentrations.

The RDA for both men and women is μg/day of dietary folate equivalents (DFEs). Factors Affecting Folate Polyglutamate Biosynthesis in Rat Liver Differential Binding of Folates but not Methotrexate (MTX) by Brush Border (BB) and Basal Lateral (BL) Membranes of Rat Renal Cortex Biosynthesis of Folate Derivatives in Ehrlich Ascites Carcinoma Cells Grown in Mice and in some Organs of the Host.

Abstract. The interrelationship between vitamin B 12 and folate metabolism in man is best illustrated by the hematologically indistinguishable, macrocytic megaloblastic anemia resulting from a deficiency of either vitamin. Large pharmacological doses of either vitamin will elicit a hematological response in patients suffering from a deficiency of either or even both vitamins (Marshall and Cited by: Español | 日本語.

Summary. Water-soluble biotin is an essential cofactor to enzymes in intermediary metabolism and a key regulator of gene expression. (More information) Both parenteral nutrition devoid of biotin and prolonged consumption of raw egg white have been associated with symptoms of frank biotin deficiency, including hair loss, dermatitis, and skin rash, ataxia, seizures, and.

The International Symposium on the Chemistry and Biology of Pteridines and Folates is a forum for presenting recent and exciting advances in this expanding field. In of ideas results that has often stimulated bringing together various disciplines, a synergy fresh approaches to major problems.

Threlfall et al. () observed that parenteral administration of mg folate/kg of BW causes a renal hypertrophy in the rat. This effect is not seen in the villi of the small bowel (Tilson, ), and it is likely that the renal effect is due to tubular obstruction rather than to any meta- bolic effect.

Folate transport mediated by human, mouse and rat PCFTs is electrogenic, indicating that there is a net translocation of positive charges as each folate molecule is transported (7,59,66,67).

Folate-induced currents in Xenopus oocytes microinjected with PCFT cRNA increase as the pH is decreased in parallel to increased transport of tritiated.

Folic acid is an N-acyl-amino acid that is a form of the water -soluble vitamin B9. Its biologically active forms (tetrahydrofolate and others) are essential for nucleotide biosynthesis and homocysteine remethylation. It has a role as a B vitamin, a human metabolite, a nutrient and a mouse metabolite.

It is a member of folic acids and a N-acyl. B vitamins are the precursors of essential metabolic cofactors but are prone to destruction under stress conditions.

It is therefore a priori reasonable that stressed plants suffer B vitamin deficiencies and that certain stress symptoms are metabolic knock-on effects of these deficiencies. Given the logic of these arguments, and the existence of data to support them, it is a shock to realize Cited by: In silico modeling of the folate biosynthesis pathway showed that these observations are consistent with the in vivo cleavage of 5,methylene-THF by a side-activity of PanB, with FolK-mediated recycling of the pterin cleavage product, and with regulation of folate biosynthesis by folates or their damage products.}, doi = {/fmicb   Investigations into the biochemical processes and regulatory mechanisms of nitrogen (4274[1]) utilization can aid in understanding how 4274[1] is used efficiently in plants.

This report describes a deficiency in 4274[1] utilization in an Arabidopsis (Arabidopsis thaliana) transfer [DNA][2] insertion mutant of the mitochondrial folylpolyglutamate synthetase gene DFC, which catalyzes the Cited by: Introduction.

The coenzyme forms of folic acid function in the metabolism of single-carbon groups involved in the biosynthesis of pyrimidines, purines, serine, and methionine (38,50,57,).Folate deficiency can result from nutritional deficiency, malabsorption (celiac disease, inflammatory bowel disease, alcoholism, etc), medications, and diseases involving increased cell turnover (hemolytic.

Nature, ISSN, VolumeIssuepp. - The purpose of this work is to review the published studies on the mechanisms of action and resistance of 5-fluorouracil.

The review is divided into three main sections: mechanisms of anti-tumor action, studies of the resistance to the drug, and procedures for the identification of new genes involved in resistance with microarray by: Luka Z, Pakhomova S, Loukachevitch LV, Egli M, Newcomer ME, Wagner C. 5-Methyltetrahydrofolate is bound in intersubunit areas of rat liver folate-binding protein glycine N-methyltransferase.

Biol. Chem. Macfarlane AJ, Liu X, Perry CA, Flodby P, Allen RH, Stabler SP, Stover PJ. Neural tube defects (NTDs) are common complex congenital malformations resulting from failure of the neural tube closure during embryogenesis.

It is established that folic acid supplementation decreases the prevalence of NTDs, which has led to national public health policies regarding folic acid.

To date, animal studies have not provided sufficient information to establish the metabolic and/or Cited by: Nazario GM, Lovatt CJ. Separate de novo and salvage purine pools are involved in the biosynthesis of theobromine but not caffeine in leaves of Coffea arabica L.

Plant Physiol. Neymeyer V, Tephly TR, Miller MW. Folate and formyltetrahydrofolate dehydrogenase (FDH) expression in the central nervous system of the mature rat.

@article{osti_, title = {Cobalamin inactivation by nitrous oxide produces severe neurological impairment in fruit bats: protection by methionine and aggravation by folates}, author = {van der Westhuyzen, J and Fernandes-Costa, F and Metz, J}, abstractNote = {Nitrous oxide, which inactivates cobalamin when administered to fruit bats, results in severe neurological impairment leading to.

Folate, the essential vitamin that fuels the one-carbon cycle for the biosynthesis of nucleotide and amino acid building blocks, was one of the first natural products found to contain pterin. (1, 2) The metabolic importance of pterins is illustrated by their integration into enzyme cofactors such as molybdopterin and : Antonio Angelastro, William Michael Dawson, Louis Y.

Luk, E. Joel Loveridge, Rudolf K. Allemann. Membrane transporters and folate homeostasis: intestinal absorption and transport into systemic compartments and tissues - Volume 11 - Rongbao Zhao, Larry H. Matherly, I.

David GoldmanCited by:   eBook is an electronic version of a traditional print book THIS can be read by using a personal computer or by using an eBook reader. (An eBook reader can be a software application for use on a computer such as Microsoft's free Reader application, or a book-sized computer THIS is used solely as a reading device such as Nuvomedia's Rocket eBook.

Van der Laken reported on the preclinical characterization of an FR-targeted PET tracer, [18 F]-PEG-folate, in an arthritic rat model [, ]. [18 F]PEG-folate imaging demonstrated good and specific visualization of inflamed joints in arthritic rats and had a lower background uptake than other common macrophage tracers, e.g., [11 C]PKCited by: 8.

Tetrahydropterin-dependent aromatic amino acid hydroxylases (AAHs) are known from animals and microbes but not plants. A survey of genomes and ESTs revealed AAH-like sequences in gymnosperms, mosses, and algae.

Analysis of full-length AAH cDNAs from Pinus taeda, Physcomitrella patens, and Chlamydomonas reinhardtii indicated that the encoded proteins form a distinct clade Cited by: The role of folylpolyglutamates in biosynthetic processes has gained increasing importance with the recognition that these com­ pounds are not only the forms of folate co-factors that accumulate within cells, but, in addition, are the preferred substrates for folate dependent reactions in eukaryotic cells.

You can write a book review and share your experiences. Other readers will always be interested in your opinion of the books you've read. Whether you've loved the book or not, if you give your honest and detailed thoughts then people will find new books that are right for them., Free ebooks since   The soluble folate-binding proteins of serum and milk are high-affinity folate receptors that have been released from cell membranes by proteolysis,82 These proteins can be detected in approximately 15 percent of normal individuals83 and are found at increased levels in some pregnant women, women on oral contraceptives, folate-deficient.

(C) Folate deficiency induces DHF depletion and enhances the transactivational potential of E2F1. (D) Excess of dTTP inhibits E2F1-mediated apoptosis and activates Chk1 in melanoma cells.

High levels of DHFR and TS could reactivate de novo dTMP biosynthesis impeding depletion of dTTP. Excess of dTTP would prevent apoptosis by several by: 4. Methotrexate is an antimetabolite and antifolate agent with antineoplastic and immunosuppressant activities.

Methotrexate binds to and inhibits the enzyme dihydrofolate reductase, resulting in inhibition of purine nucleotide and thymidylate synthesis and, subsequently, inhibition of DNA and RNA syntheses. Methotrexate also exhibits potent immunosuppressant activity although the mechanism(s) of. Shareable Link.

Use the link below to share a full-text version of this article with your friends and colleagues. Learn more. INTRODUCTION. The compounds of the folate family, consisting of folic acid, 5-methyltetrahydrofolate (5-MTHF) and other derivatives, are vitamins that act as coenzymes in reactions of 1-carbon are required for purine and pyrimidine biosynthesis and for the conversion of homocysteine to methionine, among other reactions (Stover, ).Cited by:   eBook is an electronic version of a traditional print book THIS can be read by using a personal computer or by using an eBook reader.

(An eBook reader can be a software application for use on a computer such as Microsoft's free Reader application, or a book-sized computer THIS is used solely as a reading device such as Nuvomedia's Rocket eBook.).

Introduction. Folate (vitamin B 9) is an essential nutrient for embryonic development and the maintaince of proper physiological serves as a one-carbon carrier in cells and is heavily involved in many fundamental metabolic reactions, including the biosynthesis of purine, choline, amino acids and neurotransmitters and methyl-group biogenesis.

Consequently, there will be a decrease of DNA and RNA synthesis. In addition, MTX-polyglutamates are also inhibitors of other folate-requiring enzymes such as: TS and two enzymes related with de novo purine synthesis - glycinamide ribonucleotide transformylase (GART) and aminoimidazole carboxamide ribonucleotide transformylase (AICART).Cited by: 6.

MTX enters the cells via reduced folate carrier (RFC) or via the membrane folate binding protein (FBP) and is polyglutamated by folylpolyglutamate synthetase (FPGS) in MTXpolyglutamate, which is retained in cells for longer periods compared with MTX [31,32].

The main target of MTX and MTX-polyglutamate is the inhibition of DHFR enzyme, leading to. see more details of polyglutamates of folic acid; STIFEL, F.

B., HERMAN, R. H. and ROSENSWEIG, N. S. (), effect of diet and oral folate on folate-metabolising enzymes enzymes Subject Category: Chemicals and Chemical Groups see more details in rat jejunum jejunum Subject Category: Anatomical and Morphological Structures see more details.VITAMINS AND MINERALS.

Definitions: Dietary supplement - regulatory term. Includes vitamins, minerals, herbs, botanicals, fatty acids, and amino acids as long as they are prescribed in dosage forms, such as capsules, tablets, liquids, gels or powders.The effect of dietary levels of folate and cobalamin on the serum concentrations of folate and cobalamin in the dog.

J. Nutr. S. Davey, R.J., and J.W. Stevenson. Pantothenic acid requirement of swine for reproduction.